Clinical significance of LncRNA-MIAT as a non-invasive diagnostic marker of non-Hodgkin's lymphoma associated with occult hepatitis C virus infection
Authors
- Nearmeen Rashad 1
- Sameh Abdelazeem Soliman 2
- Ahmed Ali Obaya 3
- Abdelmonem Mohamed Elshamy 4
- Amira M. El Helaly 5
- Marwa H.S Hussien 6
- Ahmed Fathy Gomaa 7
1 zagazig
2 Internal medicine department, faculty of medicine, zagazig university.
3 Clinical Oncology and Nuclear Medicine department, faculty of medicine, Zagazig University
4 Tropical Medicine medicine department, faculty of medicine,zagazig university
5 Clinical pathology department, faculty of medicine,zagazig university
6 Medical Biochemistry Department, faculty of medicine,zagazig university
7 Internal medicine department, faculty of medicine,zagazig University
Abstract
Aims: the current research aimed to investigate LncRNA-MIAT in patients with non-Hodgkin lymphoma (NHL) and to assess its correlation with clinicopathological features and treatment protocols of NHLs among Egyptian patients with Occult hepatitis C virus (HCV) infection (OCI).
Patients & Methods: This study was conducted on 20 patients with NHL and 30 healthy subjects as the control group. All subjects were screened for HCV-RNA in both plasma and PBMCs. RT-PCR determined lncRNA-MIAT.
Results: lncRNA-MIAT relative expression level was upregulated in NHL groups (2.73±0.86) compared to controls (1.06±0.07), P ˂0.001*. Among NHL, patients with OCI (3.2±0.63) had significantly higher levels of lncRNA-MIAT compared to HCV (2.6±1.08) and non-HCV (2.4±0.4), P ˂0.001*. Additionally, the relative expression levels of lncRNA-MIAT were significantly positively correlated with laboratory and clinicopathological features of NHL. Interestingly, concerning the treatment of DLBCL-NHL, there were significantly higher levels of lncRNA-MIAT in no treatment subgroup (n=10, 3.31±0.95) compared to successfully treated subgroups [CHOP (n=7, 1.58±0.34) and R-CHOP (n=3, 11.16±0.21), P ˂0.001*
Patients & Methods: This study was conducted on 20 patients with NHL and 30 healthy subjects as the control group. All subjects were screened for HCV-RNA in both plasma and PBMCs. RT-PCR determined lncRNA-MIAT.
Results: lncRNA-MIAT relative expression level was upregulated in NHL groups (2.73±0.86) compared to controls (1.06±0.07), P ˂0.001*. Among NHL, patients with OCI (3.2±0.63) had significantly higher levels of lncRNA-MIAT compared to HCV (2.6±1.08) and non-HCV (2.4±0.4), P ˂0.001*. Additionally, the relative expression levels of lncRNA-MIAT were significantly positively correlated with laboratory and clinicopathological features of NHL. Interestingly, concerning the treatment of DLBCL-NHL, there were significantly higher levels of lncRNA-MIAT in no treatment subgroup (n=10, 3.31±0.95) compared to successfully treated subgroups [CHOP (n=7, 1.58±0.34) and R-CHOP (n=3, 11.16±0.21), P ˂0.001*
Conclusions: lncRNA-MIAT level was upregulated in NHL patients, particularly patients with OCI. Thus, circulatory lncRNA-MIAT may serve as a promising non-invasive diagnostic marker for NHL associated with OCI.
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